The main goal of this course is to give an introduction into the broad range of modern biophysical methods (SPR, FCS, CD, ITC, AUC, DLS/SLS, NMR, MS, Cryo-EM, single molecule spectroscopy, DSF, native gel electrophoresis) used to study the different aspects of protein – ligand interactions

The study of interactions between proteins and other cellular partner molecules is fundamental to the understanding of biological systems. Drastically increased interest in this problem among investigators working in different fields of biological science needs to be supported by knowledge of biophysical tools and approaches dealing with protein interactions. It is an attractive idea to bring together excellent biophysicists and students to exchange experiences and to discuss the philosophy and practical elements related to the study of protein-ligand interactions.

The practicals  will cover a broad range of modern biophysical methods :

Isothermal Titration Calorimetry

Circular Dichroism

Surface Plasmon Resonance

Dynamic and Static Light Scattering

Native Electrophoresis

Fluorescence based methods

The course provides a forum for open and active discussion about the use of biophysical data to solve problems in the fields of cell biology, systems biology and structural biology and for drug discovery projects.

Among other things, the course will address in-depth analysis of the capabilities and restrictions of different biophysical methods leading to the development of an optimal strategy to study protein-ligand interactions and mathematical treatment of experimental data.

Additionally, the students will be introduced to the bioinformatic tools used to predict interaction partners, and to new technologies such as double polarization interferometry and thermophoresis.

We look forward to welcoming you to Oulu!

The Organisers

Co-Sponsors