Practical Course

European Molecular Biology Organization

MicroRNA-profiling

From in-situ hybridization to

 

next-generation sequencing

 

21 - 27 May, 2011 | NUI Galway| Galway| Ireland

About the Practical Course

 

MicroRNAs (miRNA) are short, noncoding regulatory RNAs involved in development, differentiation, and metabolism of most eukaryotic organisms. Investigating miRNA expression in these cellular processes is important for our understanding of fundamental biological processes and many different diseases. The course is designed to introduce researchers to the theoretical and practical aspects of the methods used for discovery and profiling of miRNA expression such as in-situ hybridization, microarray and qPCR analyses, fluorescent sorting and new-generation sequencing.

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MicroRNAs (miRNA) are short, noncoding regulatory RNAs involved in development, differentiation, and metabolism of most eukaryotic organisms. Investigating miRNA expression in these cellular processes is important for our understanding of fundamental biological processes and many different diseases.

 

The course is designed to introduce researchers to the theoretical and practical aspects of the methods used for discovery and profiling of miRNA expression such as in-situ hybridization, microarray and qPCR analyses, fluorescent sorting and new-generation sequencing.

 

The course will be focused on the theoretical and practical aspects of methods used for discovery and profiling of miRNA expression such as in-situ hybridization, microarray and qPCR analyses, fluorescent sorting and new-generation sequencing.

 

The practical part will also include data analysis, miRNA target prediction, correlation of mRNA and miRNA expression profiles as well as correlation of data obtained by various methods.

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

During the course we will pay particular attention to following aspects:

• Good scientific and laboratory practice in experimental design, preparation of samples and their quality assessment as well as critical evaluation of data;
• Introduction and explanation of different approaches and instrumentation systems used for miRNA profiling;
• Detailed troubleshooting;
• Comparison of analysis strategies and evaluation of obtained data;
• Importance of standardized approach to miRNA expression profiling and suitable normalization strategies.
 
 

The course is open to students at an advanced stage of their post-graduate work (Ph.D training) and post-doctoral fellows. 

 

 

We look forward to welcoming you to Galway!

The Organisers
 
   

 

 

 

 

 

 

 

 

 

European Molecular Biology Organization | Meyerhofstrasse 1 | 69117 Heidelberg | Germany