Practical Course

European Molecular Biology Organization

 

Two-Photon Imaging of Brain Circuits

3 - 10 September, 2011 | Munich | Germany

Programme

 

Saturday, September 3rd
16:00 - 16:30 Arrival, registration
16:30 - 17:00 Welcome talk
17:00 - 18:30 Short talks by participants (12x5min)
18:30 Reception, dinner (Institute)
 
Sunday, September 4th
09:00 - 10:30 Morning lecture: Arthur Konnerth/Nathalie Rochefort
"In vivo two-photon microscopy: basics and applications"
10:45 - 12:00 Presentation of the set-ups, animal preparation
12:00 - 13:00 Lunch (Institute)
 
  Practical work
13:00 - 17:00 In vivo calcium imaging, Multi-cell bolus loading, imaging of cortical neurons and astrocytes
17:00 - 18:00 Lecture: Fritjof Helmchen
"Principles of two-photon microscopy and calcium imaging"
   
18:00 Dinner, Biergarten
 
Monday, September 5th
09:00 - 10:15 Morning lecture: Jens Eilers
"Synaptic calcium signaling in micro- and nanodomains"
10:45 - 12:00 Morning lecture: Jason Kerr
"Imaging activity from neuronal populations in the awake and freely moving rat"
12:00 - 13:00 Lunch (Mensa, Campus of the Institute)
 
13:00 - 18:00 Practical work
In vivo calcium imaging, Multi-cell bolus loading, data analysis
   
18:00 - 19:00 Dinner
19:00 - 20:00 Wrap-up session
     
Tuesday, September 6th
  09:00 - 10:15 Morning lecture: Valentina Emiliani (Eirini Papagiakoumou)
"Advanced optical methods for the spatiotemporal control of neuronalactivity"
10:45 - 12:00 Morning lecture: Dirk Trauner
"Using Photoswitchable Ligands to Control Neural Circuitry"
12:00 - 13:00 Lunch (Mensa, Campus of the Institute)
 
13:00 - 18:00 Practical work
In vivo two-photon calcium imaging in the cortex of transgenic animals (genetically-encoded calcium indicators), chronic recordings preparation. Demonstration: In vivo imaging of mitochondrial dynamics in cortical neurons. In vivo imaging of the injured spinal cord.
   
18:00 - 19:00 Dinner
19:00 - 20:00 Wrap-up session
     
Wednesday, September 7th
  09:00 - 10:15 Morning lecture: Na Ji
"A clearer view - use adaptive optics to improve brain imaging"
10:45 - 12:00 Morning lecture: Thomas Misgeld
"Using 2-photon imaging to understand spinal cord pathology"
12:00 - 13:00 Lunch (Mensa, Campus of the Institute)
 
13:00 - 18:00 Practical work
In vivo two-photon calcium imaging in the cortex of transgenic animals (genetically-encoded calcium indicators), chronic recordings preparation. Optogenetic: channelrhodopsin.
   
18:00 - 19:00 Dinner
19:00 - 20:00 Wrap-up session
     
Thursday, September 8th
  09:00 - 10:15 Morning lecture: Olga Garaschuk
"In vivo imaging of microglia"
10:45 - 12:00 Morning lecture: Angus Silver
"Monitoring fast neuronal signaling in 3D with an Acousto-Optic Lens 2-photon microscope"
12:00 - 13:00 Lunch (Mensa, Campus of the Institute)
 
13:00 - 18:00 Practical work
Single-neuron and dendrites imaging
   
18:00 - 19:00 Dinner
19:00 - 20:00 Wrap-up session
     
Friday, September 9th
  09:00 - 10:15 Morning lecture: Michael Häusser
"Dendritic computation"
10:45 - 12:00 Morning lecture: Mark J. Schnitzer
"Imaging neuronal calcium activity in behaving animals"
12:00 - 13:00 Lunch (Mensa, Campus of the Institute)
 
13:00 - 18:00 Practical work
Single-neuron and dendrites imaging
   
18:30 Gala dinner
     
Saturday, September 10th
  09:00 - 11:00 Concluding brief presentations by the students, data back-up, general discussion of the results
11:15 Departure

 

 

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