Topics

1. Sample handling and protein/peptide enrichment strategies.
2. Peptide and protein analysis by MALDI-MS and MS/MS technologies, including sample preparation.
3. Protein analysis and peptide sequencing by ESI-MS and LC-MS/MS.
4. MS/MS fragmentation of peptides, e.g.CID, HCD and ETD
5. Analysis of post-translational modifications by MS, including phosphopeptide enrichment and sequencing techniques.
6. MS-based quantification strategies in Proteomics.
7. Computational data-analysis, including database searches, de novo sequencing, data validation and bioinformatics analysis of large datasets. 

Performing mass spectrometry based proteomics requires background knowledge and experience. In this course we therefore aim to provide an overview of state-of-art techniques for the analysis and characterization of protein and peptide samples by ESI and MALDI Mass Spectrometry and tandem mass spectrometry (MS/MS).

We provide the participants with knowledge on sample handling, protein and peptide enrichment and separation procedures, fragmentation techniques and we describe and demonsrate the various instrument types. We will also focus on how to analyse post translational modifications, such as acetylation, glycosylation and phosphorylation and how to quantify proteins and peptides in complex samples. Data interpretation is of course an essential part of any MS based proteomic study and will also be covered.

The course will provide the participants with hands-on experience on mass spectrometry analysis and associated data analysis and our students and researchers will give tips and hintsf on various aspects of the daily life in a proteomics research llab.

The course is aimed at cell biologists, biochemists, bioinformaticians and others with an interest in modern mass spectrometry for protein and peptide analysis in Proteomics.