ABOUT THE Practical Course
This EMBO Practical Course is aimed at young scientists working in the field of structural biology including biochemists, biophysicists, and those specializing in other structural techniques (macromolecular crystallography, electron microscopy, NMR) and in bioinformatics. The Сourse participants are encouraged to bring their own samples to perform synchrotron solution scattering experiments on-site.
Small-angle scattering (SAS) of X-rays and neutrons (SAXS/SANS) yields low resolution models of biological macromolecules in solution and also allows one to rapidly analyze structural changes in response to variations in external conditions.
The focus of the modern structural biology has recently been shifted from individual macromolecules to complex objects, molecular machines and processes, i.e. towards a systemic approach. The major task for structural methods in this systemic approach is to elucidate the structural mechanisms behind the interactions to find out how do these mechanisms give rise to the functional behaviour of the system.
SAS appears to be ideally positioned for the structural systems biology applications, being able to rapidly screen a broad variety of the experimental conditions (temperature, pH, salinity, ligand addition etc). The extremely broad range of molecular sizes accessible by SAS, from kDa to GDa, allows one to simultaneously monitor the interactions in hierarchical systems at different levels of structural organization.
The last years saw a renaissance of SAS in structural biology, thanks to the new data analysis methods and a broader accessibility of high brilliance instruments on large scale facilities (particularly, synchrotrons), which allow for high throughput SAXS with minimum amounts of material. The course participants are encouraged to bring their own samples to perform synchrotron solution scattering experiments on-site and the results will be used for practical tutorials and discussed on the last day of the course.
There will be an additional possibility for a combination of SAXS with sample optimization and crystallization experiments, supported by the EU program P-Cube. The selected participants will be offered the opportunity to send their samples in advance to the EMBL Hamburg high-throughput crystallization platform. Depending on the crystallization results, P-Cube may sponsor optimization of the sample conditions, use of the EMBL Sample Preparation and Characterization facility and more extensive SAXS experiments during the course. Please contact Rob Meijers for more information.
European Molecular Biology Laboratory (EMBL), Hamburg outstation
We look forward to welcoming you