Appreciation of RNA 3' end formation as a key regulatory step in gene expression has increased dramatically in recent years. We will start with transcription by considering how the chromatin template is made ready for transcription initiation and how the end of the gene is connected to its beginning. This will lead into how processing of pre-mRNA is coupled to transcription elongation and how splicing is regulated via specific chromatin marks. More importantly transcription coupled 3' end processing of mRNA, small stable RNAs (snRNA, snoRNA), cryptic unstable RNAs in yeast (CUTs) and other non-coding RNAs in higher organisms will be discussed. Transcripts of well-over 50% of human genes are now known to undergo alternative polyadenylation (APA.). We will examine the underlying mechanisms, and the important role played by APA in cell differentiation and disease. Here the impact of genomic analysis is especially dominant since most transcripts display variable 3' ends, conferring different regulatory properties on mRNAs. Superimposed over productive transcription, many coding and non coding RNAs follow the same expression pathways but are somehow distinguished from functional RNA and subjected to rapid degradation. This selection process will also be extensively covered during the workshop.





Brasenose College
Radcliffe Square
Oxford OX1 4AJ
United Kingdom

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