ABOUT THE PRACTICAL COuRSE
This course will focus on advanced quantitative fluorescence microscopy techniques used for imaging a range of biological specimens. The course is designed for biophysicists, or cell/molecular biologists with experience in light microscopy wanting to start using advanced fluorescence microscopy methods in their own research labs.
The participants will acquire a theoretical understanding and hands-on experience of state-of-the-art equipment for advanced fluorescence microscopy, including:
(structured illumination or SIM, photoactivated localization
microscopy or PALM, and stimulated emission depletion microscopy or STED), fluorescence fluctuation methods (number and brightness or N&B,
raster scanning correlation spectroscopy or RICS), fluorescence lifetime imaging microscopy (FLIM) and single-molecule tracking.
During practical sessions, students will learn:
(1) how to design and implement imaging experiments, such as choosing (photoactivable) fluorescent proteins, or working with live samples requiring environmental control;
(2) digital image processing and analysis;
(3) how to prepare specimens for microscopy (such as how to fix cells for STORM experiments);
(4) how to trouble-shoot common problems that occur in the course of an experiment.
Arnaud de Villeneuve Campus Montpellier
We look forward to