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Biomolecular interaction analysis 2016: From molecules to cells

7 – 11 November 2016 | Porto, Portugal

Registration deadline

20 July 2016

Abstract submission deadline

30 September 2016 (after acceptance only)

Selected participants will be notified by

27 July 2016

Payment deadline

15 September 2016

Apply now!

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About the practical course

A comprehensive and integrated characterization of molecular interactions is key to the understanding of life processes at a molecular level. Due to their complexity the study of binding events requires orthogonal approaches, i.e. the gathering of data from multiple methods in order to build a complete picture of their function.

Hence this practical course aims at a multidisplinary approach to the study of molecular interactions from and in biological systems, integrating biochemical/biophysical methodologies with live microscopy techniques.

In this 3rd Edition the course will cover a range of methods used in the kinetics and thermodynamics characterization of binding events, such as:

  • Isothermal Titration Calorimetry (ITC)
  • Surface Plasmon Resonance (SPR)
  • Microscale Thermophoresis (MST)
  • Electro-Switchable Biosurfaces (switchSENSE)
  • Biolayer Interferometry

The bridge to in vivo will be crossed with the introduction of advanced light microscopy techniques, e.g.:

  • Fluorescence Resonance Energy Transfer (FRET)
  • Fluorescence Lifetime Imaging Microscopy (FLIM)

to study the dynamic interactions of proteins within the living cell.

Practical sessions

The practical sessions will be organized in 5 rotations of 5 elements. Each group will rotate through sessions focused on the topics of the course.

Each practical session will start with a short introduction to the equipment followed by the presentation of the scientific background and objective of the experimental work, and the experiments started (part A). The results obtained will be then processed, analyzed and discussed with the tutors (part B).

Participants samples and data

Participants will be invited to:

a) propose hypothesis/questions within their work that could be addressed using one or more of the techniques focused in the course; or

b) bring data previously acquired to process, analyze and discuss (troubleshooting).

After a feasibility assessment applicants will be invited to bring their samples to assay and/or data to analyze.

Poster Sessions

Successful applicants who attend the course will be requested to bring a poster describing their project. Posters will be on display from the first day and 2 poster sessions will be organized at days 2 and 3.







Registration deadline

20 July 2016

Abstract submission deadline

30 September 2016 (after acceptance only)

Selected participants will be notified by

27 July 2016

Payment deadline

15 September 2016












Registration includes:

  • Accommodation in single room for 6 nights
  • Meals and coffee breaks
  • Social events and transfer to and from social events
  • Course material and reagents


Payment details will be sent to selected applicants via email.


The social programme will include a sightseeing tour, a visit to the Port Wine Cellars and a gala dinner at day 3 (Wednesday).

Selection Criteria

Participants will be selected according the research interests, level of knowledge and potential use of the techniques in view of their project work as specified in a motivation letter. Additionally, a fair gender balance will be considered and significant regional bias will be avoided

Please submit the Application Form, attaching your Curriculum Vitae, a motivation letter (giving a concise description of your research, your reasons for wishing to attend and your experience of techniques taught on the course) and a PDF copy of a signed Letter of Recommendation on organization letter headed notepaper from an academic referee, preferably PhD supervisor or Head of Laboratory. This letter should indicate how your research, the laboratory in which you work and the area of research that you intend to pursue will benefit from your participation on the course.

Successful applicants (maximum of 25) who attend the course will be requested to submit an abstract and bring a poster describing their project, which will be presented during 2 poster sessions and will remain on display for the duration of the course.

Abstract Guidelines

During the poster sessions participants will have the opportunity to introduce their work and to stimulate lively discussions and exchange of ideas between participants and speakers.

Abstracts are limited to a maximum of 350 words, excluding title, authors and affiliations.

Poster Specifications

The poster should be printed with the following dimensions: 90 (width) x 120 (height) cm.

Travel grants

A limited number of travel grants are available for eligible participants. Applicants do not need to apply separately for travel grants for this event. Selection of awardees is handled directly by the organizer who will notify all eligible participants. More information is available at EMBO Travel Grants' page.




Rita Matos

Phone: +351 22 0408811




venue map



IBMC - Institute for Molecular and Cell Biology
i3S - Instituto de Investigação e Inovação em Saúde
Rua Alfredo Allen, 208
4200-393 Porto, Portugal



Accommodation will be provided in single room at the Moov Hotel Porto Centro for all participants from the 6th of November (Sunday) to the 12th of November (Saturday) – total of 6 nights. The hotel is located in the Porto world heritage centre, which is within 10 min distance by public underground transportation (Metro) to the course venue and with door to door Metro connection to the city international airport.

Metro tickets to commute between the hotel and the venue will be provided.


By plane
The closest airport is the Oporto’s Airport (called Francisco Sá Carneiro Airport, OPO). Oporto’s Airport is well connected to the city center. You have the following options:

  • Shuttle Service: Direct connection between the Airport and the Hotel (average time: 20 minutes; price: 6€)
  • Metro ( You should take the E line at the airport. The destination station is Trindade then change to line D (yellow line, direction Santo Ovidio) and exit at station São Bento (average time: 23 minutes; price: 1,85 €).
  • Taxi (average time: 15 minutes; average price: 25€, however there are extra-charges for luggage and night hours).

By train
If you use the train, you may get off at Campanhã train station or São Bento train station. From both stations you can take the bus or the metro to the venue.

By metro (
i3S is located in front of Pólo Universitário Metro station. If you are coming from the city Centre, you should take line D (yellow line) – Direction São João (average time: 8 minutes; price: 1,20€).

By car
The i3S GPS coordinates are: 41º 10' 30.927'' N, 8º 36' 11.006'' W.
Please note that i3S does not offer parking space.

About the Area


Portugal's second-largest city, Porto (or Oporto) retains all the ambience of a riverside community, keeping alive ancient traditions. The town is named on UNESCO's World Heritage List, while being the country's most important commercial and manufacturing area. It is built on steep granite hills overlooking the River Douro, with a mass of 19th-century narrow streets and alleys at its heart.
The river is straddled by six dramatic bridges that connect the town with the Vila Nova de Gaia area, traditional home of the port-wine lodges and Porto's largest tourist attraction. Theatres, live music of all kinds, bars, nightclubs, cafés and restaurants all contribute to make this a thriving, dynamic city that no visitor to Portugal should miss.
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