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Structural characterization of macromolecular complexes

21 – 27 May 2016 | Grenoble, France

Registration deadline

15 March 2016

Selected participants will be notified by

15 April 2016

Payment deadline

15 May 2016

Apply now!

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About the practical course

This EMBO Practical Course aims to teach participants how to expedite structural biology projects involving macromolecular complexes by combining multiple experimental approaches. In particular, the course will cover the techniques used to produce, purify, reconstitute and characterize multi-subunit protein and protein/nucleic acid complexes for structural analysis.
Topics include: multi-subunit expression in bacteria, insect and mammalian cells; affinity, electrophoretic and centrifugation methods to isolate and purify sub-complexes and holo-complexes; biochemical and biophysical experiments that inform on the stoichiometric and conformational homogeneity of complexes; and strategies for determining the overall 3D architecture of large macromolecular assemblies and ultimately their structure at atomic or pseudo-atomic resolution.


Special emphasis will be placed on:
(i) the latest advances in isolating complexes from endogenous sources,  in recombinantly co-expressing binary and higher-order combinations of complex components, and in reconstituting complexes from individually purified components or sub-complexes;
(ii) techniques for assessing and improving sample quality (homogeneity, stoichiometry, monodispersity);
(iii) combining structure determination methods (crystallography, cryo-electron microscopy, small-angle scattering, NMR , native mass spectrometry, etc.) to obtain a multi- resolution view of a given complex.

 

Speakers

 

Programme

 

Registration

Registration deadline

15 March 2016

Selected participants will be notified by

15 April 2016

Payment deadline

15 May 2016

 

Student/postdocs

EUR200

Academic

EUR200

Industry

EUR1000

 

 

 

 

Registration includes:

  • Accommodation for 7 nights (May 20 - 27)
  • Food
  • Course materials

Selection Criteria

1. Priority will be given to advanced Ph.D students and early-stage postdocs
2. Priority will be given to applicants working on a challenging structural biology project involving a multi-subunit macromolecular complex

3. Preference will be shown for applicants (of any nationality) working in EMBC labs; a limited number of applicants based in non-EMBC Member States will also be accepted

Payment

Payment instructions will be sent to successful applicants once the selection procedure is complete.

Travel grants

A limited number of travel grants are available for eligible participants. Applicants do not need to apply separately for travel grants for this event. Selection of awardees is handled directly by the organizer who will notify all eligible participants. More information is available at EMBO Travel Grants' page.

 

 

 

Contact

Rokhaya Tounkara

Phone: +33(0)4 76 20 71 23

Email: gtounkar@embl.fr

 

Carlo Petosa

Phone: +33(0)4 57 42 86 17

Email: carlo.petosa@ibs.fr

 

 

 
venue map

Venue

venuevenue2
EPN Campus
71 avenue des Martyrs
38000 Grenoble
FRANCE

Web: http://www.epn-campus.eu

Accommodation

All participants and speakers will be lodged at the EPN Guesthouse, within walking distance of the course venue.

Travel

Details on reaching the EPN campus by train, plane or car can be found on the EPN website, here:
http://www.epn-campus.eu/getting-here/travelling-to-the-epn-campus/

Sponsors

FRISBIinstructPSB