About the practical course
3D imaging in developmental biology and mesoscopy is currently a topic of major interest for biologists, partly because of the tremendously fast pace of development of imaging technologies. These technical achievements have boosted the interest in understanding morphogenetic processes, which are still best understood only at the molecular level. Though most researchers are already familiar with the use of confocal microscopes, obtaining high quality, artifact free multidimensional images and analyzing them is still a challenge, and the expertise on novel techniques such as SPM/DSLM and Optical Tomography is still rare in most microscopy laboratories.
This EMBO Practical Course is unique in that it focuses specifically in one of the most challenging and interesting aspects of developmental biology: imaging of embryos in vivo. It is also designed to give participants the tools to obtain and analyse 3D images, something that is normally marginal in other "live cell imaging" courses.
This EMBO Practical Course is aimed mostly at researchers in developmental biology, interested in specific questions that require observation of cell movement and tissue morphogenesis, or understanding complex 3D spatial relationships between tissues, particularly difficult using conventional microscopy. Techniques such as multiphoton, SPIM/DSLM and OPT are a major feature of the course, as well as both open source and commercial solutions for equipment development and image analysis. Several commercial partners participate and contribute with state of the art techniques and equipment that are available to the participants during the course. The visualization, processing, analysis, publication and production of multidimensional images is addressed with both commercial and open source tools.