About the lecture course
Structural methods applicable to solutions offer a unique opportunity to carry out structural and functional studies on biological macromolecules under close to native conditions. We will cover the major structural and biophysical techniques for characterization of high and low resolution structure and structural transitions, macromolecular complex formation, protein folding, stability, interactions and enzymatic mechanisms. The EMBO Global Exchange Lecture Course includes lectures on:
- small-angle X-ray and neutron scattering (SAXS/SANS),
- nuclear magnetic resonance (NMR),
- static and dynamic light scattering (SLS/DLS),
- analytical ultracentrifugation (AUC),
- differential and isothermal calorimetry (DSC/ITC),
- spectroscopic approaches,
- cryo-electron microscopy (cryo-EM),
- bioinformatic tools to analyse protein-protein interactions.
Special attention will be paid to interdisciplinary approaches, where the synergistic use of complementary techniques (including macromolecular crystallography) leads to a comprehensive description of macromolecular systems.