About the practical course
This EMBO Practical Course is aimed at young scientists working in the field of structural biology including biochemists, biophysicists, and those specializing in macromolecular crystallography (MX), electron microscopy (EM), NMR or bioinformatics. Small angle scattering (SAS) of X-rays and neutrons (SAXS/SANS) allows one to study native particles and complexes and to monitor structural changes in response to variations in external conditions (temperature, pH, salinity, ligands). The method provides low resolution macromolecular shapes ab initio and is readily combined with other structural, biochemical and computational techniques in hybrid approaches.
The extremely broad range of molecular sizes accessible by SAS, from kDa to GDa, allows one to monitor interactions in hierarchical systems at different levels of structural organization.
The EMBO Practical Course participants will have the possibility to learn the basics of SAS, sample preparation, experiment design, data interpretation and will get hands-on experience with the latest modelling programs. The participants are encouraged to bring their own samples to perform synchrotron solution scattering experiments on-site, the results will be used for practical tutorials and discussed on the last day of the course.